Figure 2.

Although mutations that in yeast grown under CR conditions increase trehalose concentration prior to entry into quiescence do not alter ROS levels, they reduce oxidative damage to cellular proteins throughout lifespan. (A) Immunodetection of carbonyl groups in oxidatively damaged cellular proteins in chronologically aging wt and mutant strains. (B) The dynamics of age-related changes in intracellular ROS levels during chronological aging of wt and mutant strains. wt, tsl1Δ and nth1Δ cells were cultured in YP medium initially containing 0.2% glucose. Data are presented as mean ± SEM (n = 3–4). Abbreviations: D, diauxic growth phase; PD, post-diauxic growth phase; ST, stationary growth phase.