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Levels of independent variables investigated in the central composite rotational design
| Coded level | x 1 (mg/g) | x 2 (μl/g) | x 3 (h) | x 4 (g/g) |
| −2 | 0.36 | 0.00 | 4 | 0.33 |
| −1 | 0.52 | 2.35 | 21 | 0.50 |
| 0 | 0.68 | 4.70 | 38 | 0.67 |
| 1 | 0.84 | 7.05 | 55 | 0.83 |
| 2 | 1.00 | 9.40 | 72 | 1.00 |
x 1: amyloglucosidase (mg/g starch); x 2: pectinase (μl/g dry matter); x 3: time (h); x 4: solid to liquid ratio (g dry matter/g water)
