Fig. 4.

The effects of inhibitors of major cell signaling pathways on the expression of PAI-1 stimulated with lysoPC in vascular smooth muscle cells. Representative blots are shown in the upper panel. The intensity of the bands was densitometrically determined and normalized to that of corresponding β-actin bands. Graphic data are depicted in the lower panel. Western blot analysis of whole cell extracts from vascular smooth muscle cells treated for 6 hr with 10 µM lysoPC in the presence of calphostin C (10^-7 M, an inhibitor of protein kinase C), KT-5720 (10^-6 M, an inhibitor of protein kinase A) or genistein (10^-6 M, an inhibitor of PTK). Effect of DMBA (20 mM, a stimulator of PTK) on PAI-1 expression is also shown. ^*P < 0.05 vs DSF medium; ^†P < 0.05 vs vehicle-treated control.