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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Oral Microbiol. 2012 Mar 14;27(4):284–294. doi: 10.1111/j.2041-1014.2012.00644.x

Figure 1.

Figure 1

Expression of AbpA protein in culture supernatants of various growth conditions. Amylase ligand-binding assay of overnight culture supernatants precipitated with amylase. Bovine serum albumin as a negative control (BSA –ctrl), and salivary amylase as a positive control (Amy +ctrl). Growth conditions: defined media (DM); DM with 0.5 mg ml−1 amylase alone (A); DM supplemented with 1% starch (S); DM 1% starch + 0.5 mg ml−1 amylase (SA); DM with 1% starch grown overnight and 0.5 mg ml−1 amylase added and incubated for 15 min (SA15); brain–heart infusion (BHI). All supernatants were adjusted to have equal total protein content. AbpA protein (20 kDa band). Salivary amylase (59 kDa) used for precipitation is present in all lanes except negative control.