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Restoration of HTLV-1 production in T cells prepared from human CRM1 transgenic rats. The BAC clone harboring entire human CRM1 genome was microinjected to eggs of rat F344 strain to construct transgenic rats. T cells prepared from normal and hCRM1 transgenic rats were transformed by mixed culture with mitomycin-treated HTLV-1+ human T cell MT2. Then the amounts of p19 Gag protein in the culture medium were quantified by ELISA.
