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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Apr 3;69(5):303–311. doi: 10.1002/cm.21026

Fig. 2. The effects of mutations on the electrophoretic mobility of MCAK.

Fig. 2

Cells transfected with wild-type (WT) or mutant forms of FLAG-MCAK cDNA were synchronized in mitosis, lysed, and Western blotted with an antibody to the FLAG-tag. (A) WT and mutant S166A are compared. Actin was used as a loading control. (B) WT and mutant S628A are compared. Alpha-tubulin was used as a loading control. (C) WT and mutants S628A (lane A), S628A/S629A (lane AA), and S628D/S629D (lane DD) are compared.