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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Apr 3;69(5):303–311. doi: 10.1002/cm.21026

Fig. 3. The effect of mutations at S628 and S629 on the stability of MCAK.

Fig. 3

Cells transfected with FLAG-MCAK cDNA were incubated 16 h without tetracycline to induce expression and then lysed at varying times following re-addition of tetracycline to halt further transcription. (A) Western blots with antibodies to the FLAG tag and to actin as a loading control. The blots show the decrease in FLAG-MCAK for each of the 4 indicated cell lines with time. (B) Quantification of the data from blots similar to panel A. Band intensities were determined from fluorescence emission captured by a Storm 860 scanner and expressed as FLAG-MCAK/actin ratios. The ratio at each time point was normalized to zero time set at 100%. Averages from at least 3 independent experiments are plotted along with their corresponding standard deviations.