Figure 6.

Representative confocal micrographs of cerebral blood vessels from aged Tg2576 and wild-type mice immunolabeled for either occludin or zonula occludens (ZO)-1 (red) and counterstained for DNA (blue) with TOTO-3. Blood vessels, imaged in the neocortex and hippocampus, which exhibited strong, continuous, and linear occludin (A, C) or ZO-1 (E, G) expression were considered normal, as demonstrated in the wild type. Abnormal occludin (B, F) and ZO-1 (D, H) staining displayed punctate (white arrowheads), discontinuous or interrupted (hollow white arrows), as seen in the Tg2576 cerebrovasculature. Results are representative from three mice per group from three separate experiments. Scale bar represents 20 μm (Biron et al, 2011) (permission obtained).