Table 1. Effect of significant parameter uncertainties on the predicted energy use.

Parameter	Assumption	Change to assumption	Effect on predicted total energy use
Number of parallel fiber-Purkinje cell synapses which are silent	85% of synapses are silent after learning	Assume all synapses are active	Increases from 12.8 to 17.6 μmol ATP/g/min (38% increase)
	Synapses are silent due to absence of glutamate release	Assume synapses are silent due to absence of postsynaptic glutamate receptors in the presence of glutamate release	Increases to 13.1 μmol ATP/g/min (2% increase)
Resting membrane properties of granule cell axon	Resting membrane properties of axon different to soma, calculated from hippocampal space constant measurements	Assume resting membrane properties of axon are the same as those of the soma	Increases from 12.8 to 44.9 μmol ATP/g/min (a 3.5-fold increase)
Granule cell mean firing rate	Assume a time averaged firing rate of 3 Hz	Increase the firing rate by 1 Hz	Increases by 0.7 μmol ATP/g/min (a 1.1% change)