Fig. 5.
Effects of Dll4 blockade on insulin sensitivity and macrophage accumulation in fat tissue. (A and B) Blood glucose levels (A) and serum insulin levels (B) after 4-h fasting (n = 9). (C and D) Glucose tolerance test (GTT) after 16-h fasting and insulin tolerance test (ITT) after 4-h fasting (n = 7). (E) Quantitative RT-PCR analyses of genes related to insulin sensitivity in epididymal fat (n = 9–10). (F) Mac3 staining and population of Mac3-positive cells in epididymal fat (n = 7). (Scale bar: 100 μm.) (G) Quantitative RT-PCR analysis of F4/80 in epididymal fat (n = 9–10). (H) Quantitative RT-PCR analyses of expression of chemokines and cytokines in epididymal fat (n = 9–10). All data are from late-phase treatment. *P < 0.05; **P < 0.01; ***P < 0.001. All values are mean ± SEM.