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. Author manuscript; available in PMC: 2012 Aug 16.
Published in final edited form as: Chembiochem. 2011 Jun 20;12(12):1813–1817. doi: 10.1002/cbic.201100284

Figure 1.

Figure 1

Design and affinity measurement of synbody constructs. A) Cartoon representation illustrating six synbody constructs (SCs) screened for affinity to Grb2. SCs were designed to spatially separate the SH3-binding peptide and the SH2-binding peptide by 3, 6, 9, 12, 15, and 18 base pairs (nanometer distance given in parenthesis). B) The SCs were assayed for relative binding affinity to Grb2 using surface plasmon resonance. The cartoon image above each bar indicates the relative position of each peptide with respect to the longitudinal axis of the dsDNA helix.