Figure 5.
Mössbauer spectra (vertical bars) of DvFtn-E130A variant loaded with 16Fe/24-mer. The spectra (vertical bars) were recorded at 4.2 K in a parallel field of 50 mT (A) or 8 T (B). The solid line in A is a simulation of a quadrupole doublet with parameters ΔEQ = 2.93 mm/s and δ = 1.32 mm/s. For comparison with known carboxylate-bridged diferrous proteins, the Mössbauer spectra of reduced Methylococus MMOH (blue line) and BfMf loaded with 21Fe/24-mer (red line) recorded under the same experimental conditions are overlaid with the spectrum of DvFtn in B.