. Author manuscript; available in PMC: 2013 Jul 4.

Published in final edited form as: J Am Chem Soc. 2012 Jun 22;134(26):10822–10832. doi: 10.1021/ja211368u

Table 2.

Percents Mössbauer absorption of the Fe species detected in DvFtn and its E130A variant loaded with various sub-stoichiometric amounts of Fe

protein	Fe loading (Fe/24-mer)	% Mössbauer absorption of the Fe species^a
protein	Fe loading (Fe/24-mer)	diamagnetic diferrous	mixed valence Fe²⁺Fe³⁺	mononuclear Fe²⁺	paramagnetic diferrous
wild-type	62^b	70	0	18	0
	31	45	45	10	0
	16	20	60	20	0
	16+Na₂S₂O₄	80	0	20	0
E130A	16	0	0	0	100

A less than 5% uncertainty is estimated for the listed percent absorptions.

For the 62Fe/24-mer loading sample, approximately 12% of the absorption is attributed to small ferrihydrite clusters.