Figure 6.
SAPfl/fl.tgCreERT2-derived NKT cells assist late primary antibody responses on transfer of SAP+/+ OT-II CD4+ T cells. (A) Outline of CD4+ T cell transfer experiment. SAP−/−, SAPfl/fl.tgCreERT2 and WT control mice were treated with tamoxifen for 4 consecutive days and rested for additional 4 days before being adoptively transferred by 2 × 106 purified CD4+ T cells from SAP positive or negative OT-II mice. Twenty-four hours after cell transfers mice were immunized with NP-OVA plus αGalCer. Result of a single experiment with 3 to 5 animals per group. (B) Twelve days after immunization, NP-specific IgG antibody titers in the serum were determined by ELISA.