FIG. 13.

IFNaR2 inhibits cell growth in a TACE-dependent manner. (A, B) The indicated constructs were transfected via Nucleofection into either wild-type (TACE wt) or TACE-deficient (TACE ko) MEFs as indicated, plated into 96-well plates, and the rate of WST1 conversion to formazan was assayed 24 h later. The conversion rates were normalized to vector-transfected control (the right-most column in each panel) and plotted as percentage of this control (n=3; error bars correspond to the standard error of the mean). The IFNaR2 construct was HA-tagged, while the IFNaR2* and IFNaR2m1 constructs were tagged with both Flag (N-terminus) and HA (C-terminus), as per Fig. 1A.