FIG. 4.

IFNaR2 is cleaved in wild-type, but not in TACE-deficient MEFs. Plasmid DNA encoding IFNaR2-HA was introduced, via Nucleofection, into either wild-type (wt) or TACE-deficient (ko) MEFs. Eighteen hours later lysates were prepared and immunoblotted with anti-HA (upper panel). The filter was sequentially stripped and re-probed with anti-TACE and anti-EF2. The positions of full-length (98 kDa) IFNaR2-HA, the corresponding 58-kDa cleavage product (stub), wild-type TACE (WT), and the inactive, deleted version of TACE (ΔZn) are indicated. These immunoblots are representative of 2 replicates. MEFs, mouse embryo fibroblasts.