FIG. 7.

Complementation of KO clones with TACE induces IFNaR2 cleavage. Two clones from Fig. 6B (ko-6 and ko-5) were transiently transfected with plasmid DNA encoding TACE (T) or vector (V). Lysates were prepared and immunoblotted with anti-HA. Filters were stripped and re-probed with anti-TACE and anti-EF2. The left-most lane contains lysate from HEK293T cells overexpressing full length IFNaR2 to provide a size marker for the 58-kDa stub. Lysates from clone ko-5 were probed 2 times, and clone ko-6 lysates were probed 3 times. Representative blots are shown.