Figure 2.

Hepatic fibroblast growth factor (FGF) 21 signaling in mice treated with dietary fiber. (A) FGF21 content of liver lysates, as measured by Rat/Mouse Fibroblast Growth Factor 21 (FGF21) ELISA Kit from Millipore (Billerica, MA). Hepatic FGF21 proteins were measured by western blotting. Liver lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis, then transferred into nitrocellulose membranes. βKlotho, fibroblast growth factor receptor (FGFR) 1, FGFR3, peroxisome proliferator-activated receptor alpha (PPARα), and peroxisome proliferator-activated receptor γ coactivator protein 1 alpha (PGC-1α) were determined using their corresponding specific antibodies, shown in (B). Results were normalized with β-actin.

Notes: Mean ± standard error of the mean (n = 9/group). *P < 0.05; **P < 0.01; ***P < 0.001, dietary fiber group vs HFD group; ^#P < 0.05; ^##P < 0.01, CEL or PSY vs SCF group.

Abbreviations: CEL, cellulose; HFD, high-fat diet; PSY, psyllium; SCF, sugar cane fiber.