Figure 3.

Ectopic expression of LBD genes triggers spontaneous callus formation in vivo. (A) Phenotype of recovered Pro35S:LBD16 T1 seedlings with strong (S), intermediate (I) or weak (W) type. Ten-day-old control and Pro35S:LBD16 transgenic seedlings (upper panel) were grown on B5 medium without exogenous phytohormones for 20 days (lower panel). Bars = 5 mm. (B-D) Morphology of recovered S, I or W type seedlings overexpressing LBD29 (B), LBD17 (C) and LBD18 (D). Ten-day-old seedlings (upper panel) were grown on B5 medium without phytohormones for 20 days (lower panel). Bars = 5 mm. (E) Percentages of S, I or W type transgenic seedlings described in A-D. Total transgenic seedlings examined for Pro35S:LBD16, Pro35S:LBD29, Pro35S:LBD17 and Pro35S:LBD18were 610, 700, 620 and 480, respectively. (F) Expression of LBD16 or LBD18 in recovered Pro35S:LBD16 or Pro35S:LBD18 transgenic seedlings with S, I or W phenotypes. The relative levels (log₂) of LBD expression in LBD transgenic seedlings versus to that in control were shown as means ± SD from three biological replicates.