Figure 7.
LBD-directed callus has regeneration capacity on shoot inducing medium. (A) Callus-forming phenotype of root explants from ProXVE:LBD29 seedlings on the medium without (–) or with (+)17-β-estradiol. Root explants were cultured on the hormone-free B5 medium without or with 10 μM 17-β-estradiol for 50 days. (B) RT-PCR analysis of LBD29 expression in the root explants in A. (C) Shoot regeneration from calluses derived from ProXVE:LBD29 root explants. The calluses in A were transferred to shoot inducing medium (SIM) for 13 days and photographed. Bars = 5 mm.