Skip to main content
. 2012 May 1;22(7):1155–1168. doi: 10.1038/cr.2012.71

Figure 6.

Figure 6

Brefeldin-A, Wortmannin or Sar1H74L co-expression does not affect S-acylation and vacuolar membrane targeting of CBL2. CBL2-GFP and the plasma membrane-targeted OFP-TM23 protein were transiently expressed in N. benthamiana leaves (control). Either DMSO (solvent control), 50 μM BFA or 20 μm Wortmannin were applied to the infiltrated leaves and localization of proteins were examined in epidermal cells after 16 h of incubation. In addition, a dominant negative version of Sar1 (Sar1H74L) was co-expressed. In presence of BFA or Wortmannin, OFP-TM23 accumulates in intracellular compartments, while Sar1H74L co-expression blocks the export from the ER. In contrast, localization of CBL2-GFP is not affected. Bars in the merged pictures represent 20 μm.