Table 1.

Primer and target genes used for real-time PCR testing

	Primer sequence	Gene	GenBank
Homo sapiens	F 5′-ccgatgacgaacgagactctgg-3′ R 5′-tagggtaggcacacgctgagcc-3′	18 S rRNA	NR003286.2
Streptococcus pneumoniae	F 5′-ttgggaacggttgcatcatg-3′ R 5′-tcgtgcgttttaattccagct-3′	lytA	AJ243414.1
Salmonella spp.	F 5′-gacgttgatagtctgccatccac-3′ R 5′-caatgccgccagtctccgtgc-3′	miSl	CP002487.1
Plasmodium spp.	F 5′-gttaagggagtgaagacgatcaga-3′ R 5′-aacccaaagactttgatttctcataa-3′	18S rRNA	HQ283226.1
Rickettsia spp.	F 5′-gctatgggtataccgtcgca-3′ R 5′-caggatcttcgtgcatttctttcc-3′	gltA	JF303663
Borrelia spp.	F 5′-ctgacgctgagtcacgaaagc-3′ R 5′-gtgcatcgtttacagcgtagactac-3′	16 S rRNA	JF803950.1
Leptospira spp.	F 5′-cgctgaaatgggagttcgtatgatt-3′ R 5′-ccaacagatgcaacgaaagatccttt-3′	LipL32	JF509282.1