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X-ray spectra of wild-type His-tagged pyrazinamidase. Blue, recombinant PZAse; green, 20 mM Tris·HCl (pH 7.9); red, 20 mM phosphate buffer, 0.5 M NaCl, and 60 mM imidazole (pH 7.4); pink, eluted and concentrated proteins of soluble proteins of E. coli BL21(DE3)pLys without plasmid. The red arrow is pointing to the peak corresponding to zinc in recombinant wild-type PZAse.
