Table 2.
Effect of metal reactivation in the kinetic parameters of metal-depleted wild-type H37Rv PZAse
| Metals | Km (mM) | kcat (min−1) | Efficiency (mM−1 · min−1) | ||||
|---|---|---|---|---|---|---|---|
| Mean | 95% CI | Mean | 95% CI | Mean | 95% CI | P value* | |
| Non-chelated | 2.27 | ±0.20 | 681.16 | ±52.14 | 300.46 | ±6.22 | |
| Co2+ | 2.32 | ±0.24 | 772.89 | ±68.43 | 333.11 | ±8.81 | 0.070 |
| Mn2+ | 1.35 | ±0.07 | 448.69 | ±18.21 | 330.71 | ±6.09 | 0.056 |
| Zn2+ | 1.09 | ±0.06 | 168.28 | ±6.59 | 154.85 | ±3.93 | 0.001 |
*
P value (mean comparison test) corresponding to the comparison of the enzymatic efficiency between the reactivated metal-depleted PZAse and the non-chelated enzyme.
Enzymatic efficiency is defined as kcat/Km. The data are presented as the mean (CI) of triplicate tests. Non-chelated = wild-type PZAse non–metal-depleted.