Table 1.

The 19 steps required to correctly and safely prepare a rapid diagnostic test (RDT) (critical steps noted in boldface type)

1. Assemble new test packet, swab, buffer, pipette, lancet and gloves.

2. Put on new pair of gloves.

3. Check expiry date on package.

4. Check desiccant sachet is still dry (do not include answer in total score).

5. Write patient's name on cassette.

6. Place cassette on a level surface.

7. Clean finger with antiseptic/alcohol.

8. Allow finger to dry before pricking it.

9. Use a sterile lancet for finger prick.

10. Puncture the side of the ball of the finger.

11. Dispose of lancet in sharps bin immediately after pricking finger.

12. Collect blood with the enclosed pipette making sure to fill close to the first cross line.

13. Using the pipette, blot blood onto the pad in the smaller well.

14. Dispose of pipette in sharps container immediately.

15. Dispense 5 drops of clearing buffer into the larger well.

16. Wait 15 minutes before reading negative results.*

17. Read test results correctly.

18. Record results in CHW register.

19. Dispose of non-sharps (gloves, wrappers, alcohol swab, and desiccant) safely.

^*Positive results may be read before 15 minutes if control line has also appeared. Results should not be read after 30 minutes.