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(A) The PCR amplicons of patient 1 were analyzed on a 1.5% agarose gel electrophoresis and stained with ethidium bromide. Lane B = blood; lane BM = bone marrow; lane M = marker 100 bp; lane N = negative; lane P = positive from L. braziliensis; lane S = saliva; lane U = urine. (B) Comparison of the ITS1 gene sequences (L. brazili = L. braziliensis; L.sp 1 and L.sp 2 = Leishmania spp. sequences from cases 1 and 2, respectively).
