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In vitro mineralization assay. A, MC, EV, and Sh clones were cultured in mineralization medium for 1, 2, 3, and 4 weeks. At the end of each week, the cells/matrices were stained with Alizarin Red S. B, Alizarin Red S contents of Sh clones and controls. The contents were measured by absorbance at 405 nm at 4 weeks of culture. Error bars, S.D. of triplicate measurements. *, significantly different (p < 0.05) from MC and EV. Wk, week.
