FIGURE 5.

PKD1-Δ1–321 does not mimic the in vivo actions of WT-PKD1 to increase basal ERK and CREB phosphorylation in NIH3T3 cells. PKD1 signaling responses were examined in NIH3T3 cells because WT-PKD1 overexpression did not increase the already high levels of CREB and ERK phosphorylation in the HEK293 cell cultures used in our studies. Lysates from NIH3T3 cells that heterologously overexpress WT-PKD1 or PKD1-Δ1–321 were subjected to immunoblot analysis with the indicated antibodies. Similar results were obtained in three separate experiments.