FIGURE 5.

Myeloid cells accumulate in Kras G12D/+ cortex. Twelve-week-old control and CamKII-Cre; LSL Kras G12D/+ (Kras G12D/+) mice (n = 3) were perfused with Hanks' balanced salt solution before cortex dissection. Microglia/macrophages were enriched using Percoll gradient centrifugation from homogenized cortex. Numbers of purified live cells were counted. Flow cytometric analysis and quantification of different populations of myeloid cells were performed in enriched cells. Different populations of myeloid cells and their percentages are indicated on representative density plots. The absolute numbers of purified live cells are presented in bar graphs. Results are presented as average + S.D. Asterisks indicate statistically significant changes. A, flow cytometric analysis and quantification of macrophage (Mac1⁺ CD45^hi, R1) and microglia (Mac1⁺ CD45^mid, R2). B, flow cytometric analysis and quantification of neutrophils (Mac1⁺ Gr1^hi, M1) and total myeloid cells (Mac1⁺, M).