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PAR1 or PAR2 is not involved in NS/P-bEND cell contact induced cytokine/chemokine production. A, RT-PCR of PAR1 and PAR2 in bEND cells. B, RT-PCR of IL6, IL24, and CXCL10 mRNA in bEND cells cultured without NS/P cells (No Co-Cult), in bEND cells (Ctrl), or PAR1 knockdown bEND cells (siPAR1) after contact with NS/P cells (Co-Cult). RT-PCR of PAR1 shows the knockdown efficiency. S15 is an internal loading control. Shown here are representative data of three independent experiments.
