FIGURE 9.

NS/P cell-induced endothelial IL6 promotes glial and neural differentiation of NS/P cells. Expression of glial protein GFAP and early neuron protein TuJ1 in NS/P cells cultured under differentiation conditions in the absence (CTRL) or presence of recombinant IL6 protein (rIL6) or cultured with conditioned medium collected of NS/P-bEND cells in contact co-culture before (CM) or after (DP-CM) removal of IL6 protein by specific antibody. Actin is the internal loading control for Western blot assay.