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SPR binding profiles of anti-CEA scFv-Fc variants to immobilized mFcRn. Representative sensorgram overlays obtained with 0.5 μm of the anti-CEA ScFv-Fc variants injected over a flow cell coupled with mFcRn (∼900 RU) at a flow rate of 50 μl/min (pH 6.0 and 7.4). A, WT; B, I253A; C, H310A; D, H435R; E, H435Q; and F, H310A/H435Q. The sensorgrams were zero-adjusted and reference cell data subtracted.
