FIGURE 1.

Knockdown of CDC48 protein expression during zebrafish development and the morphologic phenotypes of CDC48-deficient embryos. A, knockdown using antisense MOs was performed in zebrafish embryos. The embryos were injected with 0.5, 1.0, or 2.5 nm cont-MO and zCDC48-MO1 and sampled 2 dpf. The levels of zCDC48 protein were analyzed by Western blotting using an antibody against zebrafish CDC48. B, quantified data from the knockdown experiment for groups of 10 embryos in three different experiments. *, p < 0.001. Error bars, S.E. C, left, cont-MO-injected embryos; right, CDC48-deficient embryos at same magnification. Embryos 48, 72, and 96 hpf are presented (bright-field microscopy). The arrow indicates a smaller eye and head.