Rescue of developmental abnormalities in zCDC48 morphants by overexpressing zebrafish CDC48 and human VCP.
A and B, expression of zebrafish CDC48 (A) and human VCP (B) in zCDC48-deficient embryos. CDC48 and VCP were detected in injected embryos after Western blotting using antibodies against zCDC48, which bind human VCP, and anti-His tag antibodies. C, phenotypes of the zCDC48-deficient embryos assessed after injecting the indicated zebrafish CDC48 and human VCP constructs. The percentage of affected embryos was determined, and the embryos were categorized as normal or abnormal, according to head and eye sizes and ventral curvature. The number of embryos is shown at the top. D, phenotypes of the zCDC48-deficient embryos shown after injecting the indicated zebrafish CDC48 and human VCP constructs. Embryos at 96 hpf are presented (bright-field microscopy). The arrow indicates a smaller eye and head.