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. 2012 May 1;287(27):23047–23056. doi: 10.1074/jbc.M111.332882

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Accumulation of polyubiquitinated proteins in zCDC48-deficient embryos. A, accumulation of polyubiquitinated proteins was detected by Western blotting. Triplicate samples of cont-MO- and zCDC48-MO1-injected embryos (10 embryos) and duplicate samples of zCDC48-MO2-injected embryos were loaded. B, cryosections were stained with an antibody that detects ubiquitinated proteins in the eyes and brain. Ubiquitinated protein-positive cells were detected using DAB substrate. Immunohistochemistry of zCDC48-MO1-injected embryos at different developmental stages (48, 72, and 96 hpf). Nucleus was counterstained by hematoxylin in right panel. Scale bars, 20 μm in brain and 50 μm in eye.