FIGURE 4.

HnRNP K is a physiological granzyme substrate. A, HeLa cells were incubated with GrA (1 μm), GrB (1 μm), GrH (0.2 μm), GrK (1 μm), GrM (1 μm) or their corresponding catalytically inactive control granzyme (SA) in the presence of SLO (500 ng/ml) for 30 min (GrB) or 16 h (other granzymes) at 37 °C. Lysates were subjected to immunoblotting using an antibody against the mid region of hnRNP K. B, HeLa cells were challenged with increasing E:T ratios of LAK cells for 4 h at 37 °C. Lysates were subjected to immunoblotting using antibodies directed against the mid region, N terminus, or C terminus of hnRNP K.