FIGURE 7.

HnRNP K protects tumor cells from LAK cell-mediated killing. HeLa cells were transfected with control siRNA or hnRNP K siRNA. Three days post-transfections, cells (3 × 10⁴ cells/well) were challenged with increasing E:T ratios of LAK cells for 8 h at 37 °C. Cells were stained with PI, and cell death of gated HeLa cells was analyzed by flow cytometry. LAK cell-specific cytotoxicity was calculated and corrected for background cytotoxicity as measured in HeLa cells at E:T ratio of 1:0. These background values were 7.41 ± 0.72% and 12.05 ± 0.36% for control tumor cells and hnRNP K-deficient tumor cells, respectively, and were subtracted from all E:T ratios. Data depicted represent the mean ± S.D. of triplicates. *, p < 0.01; **, p < 0.001.