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. 2012 May 15;287(27):22812–22821. doi: 10.1074/jbc.M112.339143

FIGURE 2.

FIGURE 2.

Expression analysis of LST1/A. A, Hek293FT were transiently transfected with LST1/A or an empty vector, lysed, and subjected to Western blotting using the newly developed antibody to LST1/A. Staining for GAPDH served as a loading control. B, expression of LST1/A mRNA in various human tissues was analyzed by quantitative RT-PCR. PCR amplifications were carried out in triplicates and mean Ct values were normalized to the GAPDH mRNA level. The dashed horizontal line indicates median from all tested tissue samples. C, expression of LST1/A in the isolated human peripheral leukocyte subsets was analyzed at the transcript level using quantitative RT-PCR (mean Ct values of triplicates were normalized to GAPDH) or at the protein level using Western blotting. Staining for Erk2 served as a loading control. D, expression of LST1/A in the indicated cell lines was analyzed as in C with GAPDH as a loading control for Western blotting. E, lysates of Hek293FT cells transfected with LST1/A and lysates of THP-1 cells were separated under non-reducing or reducing conditions and subjected to Western blotting with the LST1/A antibody.