Colocalization of ErbB4 with SUMO-1, PIAS3, and PML.
A, COS-7 cells expressing HA-tagged ErbB4 JM-a CYT-2, GFP-SUMO-1, and PML-3 in the presence or absence of PIAS3 or PIAS3C299S were stained with anti-HA (red) and anti-PIAS3 (sc-46682, blue). B, COS-7 cells expressing HA-tagged ErbB4 JM-a CYT-2, GFP-SUMO-1, and PML-3 in the presence or absence of PIAS3 or PIAS3C299S were stained with anti-HA (red) and anti-PML (sc-966, blue) antibodies. The cells were visualized by confocal microscopy with a ×63 objective.