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. 2012 May 14;287(27):23216–23226. doi: 10.1074/jbc.M111.335927

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — PIAS proteins in ErbB4-regulated transcription. HEK 293 cells were transfected with a construct encoding ErbB4 ICD2 fused to the GAL4 DNA-binding domain and pFR-luc together with constructs encoding Omni-YAP2 and FLAG-tagged PIAS proteins as indicated. Columns represent relative luciferase activity normalized to signal from the cotransfected Renilla luciferase construct (mean ± S.D.). *, p < 0.05. Expression of PIAS proteins and YAP2 were controlled with anti-FLAG and anti-Omni, respectively. Loading was controlled by reblotting with anti-HSP90.W, Western blot.