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. 2012 May 9;287(27):22701–22708. doi: 10.1074/jbc.M112.368876

FIGURE 3.

FIGURE 3.

Currents recorded from HEK293 cells expressing TRPML3 loop mutants E361A and D371A. A and B, steady-state current-voltage plots of whole-cell currents (A) and average inward current densities (B) measured in HEK293 cells transfected with the extracellular loop mutants or wild-type TRPML3 in response to 10-ms voltage steps from a holding potential of +10 mV between −200 and +100 mV in 20-mV incremental steps (normalized by cell capacitance (picofarads (pF)). Constitutively active inwardly rectifying currents were present in TRPML3(E361A)-expressing cells and were not detectable in TRPML3(D371A)-expressing cells. Shown are means ± S.E. Numbers in parentheses indicate the number of experiments. ***, p < 0.0001 (unpaired Student's t test) compared with the wild type. C and D, average inward current densities (C) and steady-state current-voltage plots (D) of whole-cell currents at −80 and −200 mV from experiments similar to the ones described for A and B and normalized to cell capacitance. Shown are means ± S.E. Numbers in parentheses indicate the number of experiments.