miR-143 inhibits lung cancer cell glycolysis, proliferation, and tumorigenesis via HK2 down-regulation.
A–C, detection of glucose consumption (A), Glc-6-P (B), and l-lactate production (C) in CRL-5803 cells with only miR-143 overexpression or with HK2 reintroduction after miR-143 overexpression. ctrl, control; G6P, Glc-6-P. D–F, cell growth assay (D), soft agar colony formation assay (E), and tumor formation in xenografts (F) of lung cancer cells CRL-5807 with or without miR-143 ectopic expression. G–I, cell growth assay (G), soft agar colony formation assay (H), and tumor formation in xenografts (I) after reintroduction of HK2 into CRL-5807 cells with miR-143 expression. *, p < 0.05, **, p < 0.01 and ***, p < 0.001.