FIGURE 10.

Expression and purification of recHPA on amylose-Sepharose column. A, HPA in yeast culture medium was concentrated by ultrafiltration 3 days after inoculation, and expression was analyzed by determining the reactivity with anti-HPA rabbit IgGs after SDS-PAGE, as described under “Experimental Procedures.” B, elution profile of recHPA on an amylose column. recHPA was purified as described under “Experimental Procedures.” C, fractions of peak 1 (fraction 63) and peak 2 (fraction 72) eluted from the amylose-Sepharose column were pooled, concentrated, and examined for recHPA by immunoreaction with anti-HPA IgGs. CBB, Coomassie Brilliant Blue.