FIGURE 11.

Reactivities of PPA and recHPA with sugar-BP probes by ELISA. PPA (A) and recHPA (B) were coated onto the wells of a polystyrene plate and reacted with various sugar-BP probes. The bound sugar-BP probes were detected by ELISA as described under “Experimental Procedures.” □, α-Man-BP; ●, α-Man-6-phosphate-BP; +, α-GalNAc-BP; ×, α-Gal-BP; △, α-NeuAc-BP; —, Lac-BP; ♢, LacNAc-BP. All experiments were carried out in triplicate and repeated three times, and representative data from assays are shown, representing the means ± S.E. (n = 3).