FIGURE 2.

Affinity chromatography of BBM components on PPA-Sepharose. A, the solubilized BBM was applied to a Sepharose 6B column. After washing with TBS-Ca containing 0.1% Triton X-100, the pass-through fractions (P) of BBM components were collected. B, the pass-through fractions were applied to a PPA-Sepharose column, and the proteins bound to PPA-Sepharose were eluted with 0.2 m Me α-Man in 10 mm TBS containing 25 mm EGTA and 0.1% Triton X-100 as E1. C, rechromatography of fraction E1 on a PPA-Sepharose column. The eluted proteins (E2) were detected by BCA assay. Similar experiments were repeated four times.