FIGURE 4.

Reactivity of PPA-bound proteins in BBM with biotin-PPA and various plant lectins. A, proteins in the solubilized BBM (B) and peak fractions eluted from the first PPA column (E1) separated by SDS-PAGE, electroblotted onto a PVDF membrane, and then probed with biotin-PPA or various biotin-lectins before and after acid desialylation (indicated by an asterisk). B, similarly, fraction E1 was reacted on a membrane with anti-SI or anti-SGLT1 antibodies and detected with HRP-labeled anti-rabbit IgGs. The color was developed with DAB/H₂O₂ as described under “Experimental Procedures.” The values on the left indicate the migration positions of the molecular mass markers (lane M). The values on the right indicate migration positions of bands I–VII. CBB, Coomassie Brilliant Blue. L-PHA, Phaseolus vulgaris lectin type L; PNA, peanut lectin.