FIGURE 5.

Interaction studies between PPA and SI or SGLT1 in BBM by ELISA. PPA was coated onto the wells of a microtiter plate and reacted with the solubilized BBM. The bound SI (A and C) and SGLT1 (B and D) were detected with each specific antibody and HRP-labeled secondary antibodies as described under “Experimental Procedures.” A and B, concentration dependence of the binding on immobilized PPA. C and D, inhibition of the binding with polysaccharides. ●, mannan; ○, galactan. All experiments were carried out in duplicate and repeated three times. The data represent the means ± S.E. (error bars) (n = 3), and results for C and D were analyzed by Student's t test. *, p < 0.01; **, p < 0.001 compared with that in the absence of polysaccharides.