FIGURE 9.

Effect of PPA on SGLT1 activity. A, time course of d-[¹⁴C(U)]Glc uptake by porcine duodenum BBMV in 100 mm NaCl (●) or KCl (○) was measured during 5–300-s incubations. B, concentration dependence of d-Glc uptake on PPA. Glc uptake by BBMV was measured after incubation for 5 s as described under “Experimental Procedures.” C, effects of PPA and lectins. PPA (20 μm, final 10 μm) and various lectins (100 μg/ml, final 50 μm) were added after preincubation of BBMV. All experiments were carried out in triplicate and repeated four times. The data represent the means ± S.E. (error bars) (n = 4 for B and C) and were analyzed by Student's t test. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with that in the absence of PPA (B and C) and that in the absence of lectin (C). MAM, Maackia amurensis mitogen.