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Purification of ligands for PPA from solubilized BBM
| Chromatography step | Protein | Yield |
|
|---|---|---|---|
| Stepwise yield | Total (BBM = 100%) | ||
| mg | % | % | |
| BBM extract | 40 | 100 | |
| Sepharose 6B precolumn | 25.2 | 63.0 | 63.0 |
| First PPA-Sepharose 6B | 0.235 | 0.93 | 0.59 |
| Second PPA-Sepharose 6B | 0.008 | 3.42 | 0.02 |
