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. 2012 May 4;287(27):23024–23033. doi: 10.1074/jbc.M112.367011

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Both diet control and exercise reduced APP-CTFβ accumulation. A, immunoblotting analysis of full-length APP and APP-CTFα and APP-CTFβ. Full-length APP and APP CTFs (CTFα and CTFβ) were detected by anti-APP C terminus antibody. β-Actin was detected as loading control. Long exposure indicated that the same film was exposed for a longer time. The asterisk indicates glycosylated full-length APP. To analyze APP CTFs in detail, two kinds of gels (5–20% polyacrylamide gradient gels and 4–12% NuPAGE Bis-Tris gels) were used. Unfortunately, we could not clarify the mobility of CTFs bands caused by phosphorylation presumably because of the gel conditions of our experiment. B, statistical analysis of full-length APP. The band of full-length APP was normalized by that of β-actin. The band density of the control was regarded as 100%, and that of other groups was relatively indicated. There was no statistically significant difference among control APP, APP-HFD, APP-HFD+Ex, APP-HFD+Dc, and APP-HFD+Ex+Dc mice (F_{(4, 10)} = 0.47, not significant). C, statistical analysis of APP-CTFβ. The band of APP-CTFβ was normalized by that of full-length APP. The band density of APP-CTFβ in APP-HFD mice was increased when compared with that in control APP mice. However, the band densities of APP-CTFβ in APP-HFD+Ex (F_{(4, 10)} = 4.27, *, p = 0.003), APP-HFD+Dc (*, p = 0.021), and APP-HFD+Ex+Dc (*, p = 0.023) mice were significantly decreased when compared with that in APP-HFD mice. There was no difference between APP-CTFβ in APP-HFD+Ex mice and that in APP-HFD+Dc mice. D, statistical analysis of APP-CTFα. The band of APP-CTFα was normalized by that of full-length APP. The band density of APP-CTFα in APP-HFD mice was increased when compared with that in control APP mice. The band densities of APP-CTFα in APP-HFD+Ex (F_{(4, 10)} = 4.36, *, p = 0.034), APP-HFD+Dc (*, p = 0.014), and APP-HFD+Ex+Dc (*, p = 0.024) mice were significantly decreased when compared with that in APP-HFD mice. There was no difference between APP-CTFα in APP-HFD+Ex mice and that in APP-HFD+Dc mice.